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KMID : 1134820100390111647
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 11 p.1647 ~ p.1653
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Extraction of Glycosaminoglycan from Sea Hare, Aplysia kurodai, and Its Functional Properties : 2. Structural Properties of Purified Glycosaminoglycan
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Yoon Bo-Yeong
Choi Byeong-Dai
Bea Dong-Won
Choi Yeung-Joon
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Abstract
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Glycosaminoglycan (GAG) was purified from polysaccharide extracted from sea hare muscle on DEAESepharose column and investigated for the functional groups, distribution of sugars, composition of disaccharide and structure of GAG. Purified GAG was composed of disaccharide above 55% of total sugar. Purified GAG showed amide I peak in 1648/㎝ and C-O stretch peak as properties of carbohydrate, amino acid peak in 1457/㎝, and peak in 866/㎝ as properties of monosaccharide by FT-IR. Fucose, N-acetylgalactosamine, N-acetylglucosamine, glucose, galactose, mannose and xylose were found in MALDI-TOF MS/MS spectra of hydrolysates by chondroitin sulfate ABC lyase and heparanase I. Purified GAG was expected to be heparan sulfate including N-acetylgalactosamine and N-acetylglucosamine above 70% of total sugar. The structure of GAG was supposed as GlyUA(2S)-GlcNS and GlyUA-GlcNS(6S) with O-linkage on protein core.
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KEYWORD
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sea hare, Aplysia kurodai, glycosaminoglycan , purification, structure
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